Grant Project Number: 2R01AI110964-06

“Aim 1. Characterize the diversity and distribution of high spillover-risk SARSr-CoVs in bats in southern China. We will use phylogeographic and viral discovery curve analyses to target additional bat sample collection and molecular CoV screening to fill in gaps in our previous sampling and fully characterize natural SARSr-CoV diversity in southern China. We will sequence receptor binding domains (spike proteins) to identify viruses with the highest potential for spillover which we will include in our experimental investigations (Aim 3). Aim 2. Community, and clinic-based syndromic, surveillance to capture SARSr-CoV spillover, routes of exposure and potential public health consequences. We will conduct biological-behavioral surveillance in high-risk populations, with known bat contact, in community and clinical settings to 1) identify risk factors for serological and PCR evidence of bat SARSr-CoVs; & 2) assess possible health effects of SARSr-CoVs infection in people. We will analyze bat-CoV serology against human-wildlife contact and exposure data to quantify risk factors and health impacts of SARSr-CoV spillover. Aim 3. In vitro and in vivo characterization of SARSr-CoV spillover risk, coupled with spatial and phylogenetic analyses to identify the regions and viruses of public health concern. We will use S protein sequence data, infectious clone technology, in vitro and in vivo infection experiments and analysis of receptor binding to test the hypothesis that % divergence thresholds in S protein sequences predict spillover potential.”

Color me shocked, but that’s the funding proposal and there’s nothing in there even approaching whatever you’re talking about. But hey, maybe you’re referring to the rejected DARPA grant proposal leaked by DRASTIC:

“THE PROPOSAL PLANNED TO INTRODUCE “KEY RBD RESIDUES” INTO LOW RISK STRAINS TO TEST PATHOGENICITY IN HUMAN AIRWAY-CELLS”

Wowie, looks like we have a hit! Rather than reading their spin though, I went and found the REJECTED grant proposal:

“We will sequence spike proteins, reverse engineer them to conduct binding assays, and insert them into bat SARSr-CoV backbones (these use bat-SARSr-CoV backbones, not SARS-CoV, and are exempt from dual-use and gain or function concerns)”

If you’re not aware, these backbones are common lab vectors which aren’t pathogenic themselves, made from different viruses. Their sequences are significantly different than either SARS-CoV or SARS-CoV-2. So, chimeric receptor/backbone pairs are used to assess viral entry into humanized cells more so than virulence. You may disagree with whether or not that’s still too dangerous of a method, but it’s a moot point here because 1. The backbones proposed here are completely different than COVID, so it can’t be the same viral agent and 2. This is a REJECTED PROPOSAL. None of this was actually done and it’s fantasy to pretend it is.

Next claim: aerosolized droplet for vaccines:

“We will complement [broad scale immune boosting with bat interferon] by coupling agonist treatments with SARSr-CoV recombinant spike proteins to boost pre-existing adaptive immune response in adult bats… we will incorporate [recombinant spike proteins] into nano particles or raccoon pox virus vectors for delivery to bats”

They’re not proposing aerosolizing whole droplets with competent SARS-CoV in them you moron, they’re basically saying “hey, you know those nasal sprays we use for the flu every year? Let’s give that to bats”.

Ooh, my favorite. No scientist with integrity says that the genome wasn’t manipulated.

You’re gonna have to tell that to the couple hundred scientists who have been studying this for a while:

“There is no logical reason why an engineered virus would utilize such a suboptimal furin cleavage site, which would entail such an un- usual and needlessly complex feat of genetic engineering. The only previous studies of artificial insertion of a furin cleavage site at the S1/S2 boundary in the SARS-CoV spike protein uti- lized an optimal ‘‘RRSRR’’ sequence in pseudotype systems (Belouzard et al., 2009; Follis et al., 2006). Further, there is no ev- idence of prior research at the WIV involving the artificial insertion of complete furin cleavage sites into coronaviruses.”

There really isn’t any evidence of manipulation at all. The backbone isn’t a standard lab construct. The cleavage site could have arisen from recombination. In the spirit of good science, I would never rule anything out, but the evidence very much supports a natural origin. Lab leak from a sample? Maybe, but that’s different than genetic engineering. For that you need stronger evidence. The strongest bit of evidence we have is the stonewalling from WIV and China, which is certainly suspicious. But, it’s unfortunately incidental and that isn’t good enough to jump to conclusions.

Try actually reading the text of these proposals before reading someone else’s spin on it.

God I would kill for 2 real friends

Oh no thanks, with the release of Ahsoka I’m filled up on fantasy for a while

Are we referring to Musk as a glitch now?

Now this is unpopular.

May god have mercy on your soul. LotR memes was one of the first communities to migrate over.

Can someone convert the measurement to bratwurst for the Europeans?

Uhh, what the hell is this article talking about? His approval has remained steady with both Republicans and all Americans, and even saw a small uptick recently..

Sounds like they’re still operating under 2020 and 2022 logic. Biden is pretty widely disliked nowadays outside his own party and 2024 should in no way be viewed as an easy Dem win.

… you have some reading to do my guy.

RFK can say he’s pro-vaccine all he wants, but he’s the chairman of probably the most well known and active anti-vaccine organization operating today. He has personally spoken, funded, and advocated for antivaxx events and movies, and is one of the biggest mouthpieces for the lie that vaccines cause autism, writing several articles and even books about it.

The EU thing is bullshit too, by the way. The US and EU have similar governing bodies for vaccine testing and approval.. And mandates? EU has similarly common childhood vaccine mandates in most major countries.

RFK is one of if not the major megaphone for the idea that trace amounts of thiomersal is driving autism rates, despite it 1. not being used in childhood vaccines since the 90s and 2. never having any link to autism whatsoever..

Saying “I’m pro-vaccine, I just believe the vaccines are causing an "autism holocaust" and we should let parents skip vaccine schedules because vaccines are actually just poison” is just being anti-vaxx but with a veneer of libertarianism.

And to be clear, I’ll entertain the libertarian arguments against mandates (they’re not convincing imo, but that’s a conversation for another day), but RFK is absolutely not arguing that position in good faith. He has inadvertently caused preventable deaths worldwide by advocating for fringe pseudoscience.

Growing evidence that governments/corporations would sooner give up seeing the goddamn sun than get off even a fraction of fossil fuel usage

See, that was my response too! Federated servers will waste a bit of energy with duplicate fetch requests, sure, but it’s a far cry from the decades of compute time wasted with adversarial validation and cryptographic mathematics. And that doesn’t even deal with the shitty coins themselves.

Was very confused why they seemed to think the concepts were the same, and thought maybe I was the idiot.

This isn’t easy to answer for a lot of reasons. People “leave Reddit” in a lot of ways. Some deleted their account. Some nuked all their comments but left the account up. Some just deleted the app. Some stopped using Reddit but will eventually return. Some JOINED Reddit specifically to watch the exodus drama. Some made bot accounts to fuck with the numbers for fun. And of course, some users joined without ever being aware there was drama at all. Looking at the change in the number of users alone won’t yield the answers.

Other useful metrics would be number of posts/comments contributed, and daily active user statistics. But again, engagement may have actually been driven upwards recently because drama is fun to be a part of and redditors are notorious keyboard warriors.

Growth of lemmy and other similar platforms is another metric to use, but that number is affected by the converse of all of the reasons I listed above as well: A lemmy account doesn’t mean they deleted Reddit. It doesn’t mean they’ll stay off it. Not to mention lemmy’s growth is likely inflated by people signing up for multiple instances due to slowdown.

tl;dr: No one is gonna have a good answer to this yet. If they say they do, it’s likely gonna be a pretty inaccurate estimate.

I remember in the early days of the Musk takeover he was mocked for talking about introducing microtransactions using Dogecoin as a way to combat bots and scraping. And it was good he was mocked, because crypto was a stupid as fuck idea for about a thousand different reasons. It’s interesting to read how a lot of people here support the idea of automatically sending a fraction of a cent to a website for each post to discourage bots and support smaller servers though.

With how prevalent the AI and data scraping conversation has become, I wonder what the view of Musk’s Twitter would be if he had stuck with that original plan instead of selling meaningless blue check marks. Probably still extremely negative, since a system like that would kill engagement overnight without SEO pumping tweets anyway… but an interesting thought experiment.

Not like the monetization scheme of blue check marks even ranks in the top 10 of terrible Musk decisions at this point. Seems like the dude is just constantly making and reversing poor choices.