573
Biochem
(mander.xyz)
you are viewing a single comment's thread
view the rest of the comments
view the rest of the comments
this post was submitted on 22 Jan 2024
573 points (99.0% liked)
Science Memes
11047 readers
3400 users here now
Welcome to c/science_memes @ Mander.xyz!
A place for majestic STEMLORD peacocking, as well as memes about the realities of working in a lab.
Rules
- Don't throw mud. Behave like an intellectual and remember the human.
- Keep it rooted (on topic).
- No spam.
- Infographics welcome, get schooled.
This is a science community. We use the Dawkins definition of meme.
Research Committee
Other Mander Communities
Science and Research
Biology and Life Sciences
- !abiogenesis@mander.xyz
- !animal-behavior@mander.xyz
- !anthropology@mander.xyz
- !arachnology@mander.xyz
- !balconygardening@slrpnk.net
- !biodiversity@mander.xyz
- !biology@mander.xyz
- !biophysics@mander.xyz
- !botany@mander.xyz
- !ecology@mander.xyz
- !entomology@mander.xyz
- !fermentation@mander.xyz
- !herpetology@mander.xyz
- !houseplants@mander.xyz
- !medicine@mander.xyz
- !microscopy@mander.xyz
- !mycology@mander.xyz
- !nudibranchs@mander.xyz
- !nutrition@mander.xyz
- !palaeoecology@mander.xyz
- !palaeontology@mander.xyz
- !photosynthesis@mander.xyz
- !plantid@mander.xyz
- !plants@mander.xyz
- !reptiles and amphibians@mander.xyz
Physical Sciences
- !astronomy@mander.xyz
- !chemistry@mander.xyz
- !earthscience@mander.xyz
- !geography@mander.xyz
- !geospatial@mander.xyz
- !nuclear@mander.xyz
- !physics@mander.xyz
- !quantum-computing@mander.xyz
- !spectroscopy@mander.xyz
Humanities and Social Sciences
Practical and Applied Sciences
- !exercise-and sports-science@mander.xyz
- !gardening@mander.xyz
- !self sufficiency@mander.xyz
- !soilscience@slrpnk.net
- !terrariums@mander.xyz
- !timelapse@mander.xyz
Memes
Miscellaneous
founded 2 years ago
MODERATORS
So, is it literally just repeatedly creating the necessary conditions and hoping the stuff will react the right way? Or is it a strict process that needs to be done just so or it’ll ruin the whole thing? Or both?
Biochem is incredibly sensitive to seemingly minor changes in conditions or procedure. A former coworker of mine had to change careers after the procedure she had to follow to run the assays for her master's thesis gave her severe RSI. She couldn't alter the procedure for ergonomics, though, because even something like changing the angle that she held the pipettor at could throw off the results.
In biopharma work, it's not at all uncommon when trying to manufacture a biologic to find a process that works reliably in the lab but doesn't give the same results when scaled up to production-size bioreactors, such that there's often a whole stage of R&D devoted to taking a procedure from the lab and reproducing it on successively larger pieces of equipment, while working out all the tweaks and adjustments needed to make things work and optimize production.
Fascinating. Do you know any good resources where I can learn more details like this? I'm doing undergrad biochem rn...
Alas, my knowledge on the topic is limited -- I work as a lab planner, and what I wrote above is most of what I've gleaned over the years of designing process development and scale-up labs. Past a point I just ooh and aah appreciatively at the big robotized bioreactor arrays my clients are putting in. Hopefully someone with a deeper background can point you in the right direction!
Yeah, kind of a little bit of both. Assuming that this is about bacterial transformation, it kind of goes like this in the lab. It's from 2012 so there are probably easier techniques with fewer steps.
And these (11:00-16:00) are the basics about bacterial transformation.