Diana Taverner from Slow Horses.

Veronica Palmer from Better Off Ted.

The Boss, MGS

Not Big Boss, the man with the eye patch and is a bad guy, the woman who taught him and the mother of Ocelot

She was such a badass woman that she inspired basically everyone around her to try to mold the world in the way they thought she saw it. Shes a great example of a woman/mother who's a badass directly because of the skills normally associated with those roles. Yeah, she can fight and kick your main characters ass, but that's mostly because she's spent time nurturing him and caring for him to the point where he emotionally cannot fight her. She regularly disarms strong, imposing men (some with superpowers like lightning channeling) by just looking at them sternly and implying she's about to call them by all 3 of their names in a disapproving tone

Time and again she does badass things while she's alive, and her death is technically the catalyst for the rest of the series as those who loved and admired her try to shape the world as she saw it, which isn't even something she herself did because of her loyalty to her country.

Oh and she's voiced by the same woman who does Pearl from SpongeBob, which is wild

Lauren Oya Olamina, from Parable of the Sower and Parable of the Talents. Strong, independent, and wise enough to know that there's strength in community.

Miss Marple.

Caska from Berserk

Joan Holloway from Mad Men- she at the very least has the evolution of a woman rising to independence while in a world built around suppression of women. A lot of characters from that show are badass ladies at different stages of independence though.

Agatha Heterodyne from Girl Genius.

And a lot of her friends too.

Appsros character in Abberation

Nest Freemark

Susan Sto Helit Aka the lady Susan Death.

Elastigirl

Tina Fey. And I don’t care that she’s not fictional. She’s a badass and needs to be recognized. But if I had to play by the rules…

Liz Lemon.

Because shes played by someone I admire.

Hachikuji Mayoi from monogatari series.

Senjougahara Hitagi from monogatari series.

Deishuu Kaik-- oops you didn't read that.