From Canadian Virtual Hospice:
The acronym “2SLGBTQ” stands for Two-Spirit, lesbian, gay, bisexual, transgender, queer (or questioning). The plus sign represents other sexual identities, such as pansexual or asexual. It’s important to remember that everyone – not just 2SLGBTQ+ people – has a gender identity, gender expression, and sexual orientation.
From Two-Spirit - Wikipedia:
Two-spirit (also known as two spirit or occasionally twospirited, or abbreviated as 2S or 2E[a], especially in Canada) is a contemporary pan-Indian umbrella term used by some Indigenous North Americans to describe Native people who fulfill a traditional third-gender (or other gender-variant) social role in their communities.