this post was submitted on 24 Sep 2025
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Hi, I took my first estradiol cypionate injection this Sunday after staring at the bottle for about a month and I've been experiencing some muscle soreness since. I just want to make sure I haven't fucked up something beyond repair, and it would be nice to talk to some humans about this rather than just following directions off the internet. I'll include my process at the bottom but if someone can tell me "no that happens to everyone, you're good" it would really help me. I understand there's a lot more that goes into DIY HRT and I'm working towards that too.

Injection process I followed for scientific rigor:

SpoilerI followed the directions as well as I understood it: using a luer lock syringe, I drew 0.1 mL from my vial using, I think it was a 22 gauge needle, after wiping it down with an alcohol pad, then realized I wasn't going to get that amount out of the syringe due to drawing it incorrectly or some other mishap, so I drew more of the solution, about 1.5 mL. I swapped the needle out for a 25 gauge, 1.5 inch needle which was recommended for intramuscular injections, then I stared at the needle for about ten minutes trying to keep my hands from shaking due to a currently very inconvenient phobia of them, then injected the solution into basically the meatiest part of my glute. I'm underweight, so I assume my options for injections are fairly limited. No bleeding occurred, and the injection site looks like a small red pock currently

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[–] chug-capture-ahoy@piefed.social 2 points 10 hours ago

Try to avoid using Syringes and needles you find in the local park.

[–] Cassa@lemmy.blahaj.zone 5 points 1 day ago (1 children)

(I'll start off by saying no expert here - began this year myself)

I do Subcutaneous injections and I can get a stiff sore spot - that looks red and can be felt. it goes away around after a week.

I might be misreading but how much did you inject? 1.6ml? was there any air or bubbles inside the syringe when you injected?

[–] mossy_@lemmy.world 3 points 23 hours ago (1 children)

I used a calculator on the internet to see how much solution I would need to inject to get the desired dose and I believe it was 0.1 mL. Due to the size of my syringe, when I drew that amount the first time I wasn't able to get any of the solution out of it, I depressed the plunger to the end just getting the air out. So I drew a bit more and some of it stayed in the syringe. I probably ended up with a little more than I had planned but not 60% more

[–] Witchfire@lemmy.world 5 points 12 hours ago* (last edited 12 hours ago)

I've been doing this shit for years. Here is my process

  1. Wash your hands thoroughly
  2. Clean vial and skin with iso
  3. Fill syringe with air (I'd say about double your dose)
  4. Stab into vial and inject air into vial
  5. Flip upside down and draw dose
  6. Take out, draw air into the syringe
  7. Swap out your needles
  8. Push out air until a bead forms at the tip
  9. Stabby stab and push
  10. Treat with alcohol pad and bandaid
  • I know some people purposefully use an air bubble to push out the full amount but that's riskier so I don't recommend it
  • Soreness is normal. Sometimes it happens sometimes it doesn't.
  • Don't EVER reuse needles and dispose of them in a hard plastic container (get a sharps container if you don't have one)
  • Disclaimer: I'm not medically trained, I'm just a trans kinkster and sideshow performer
[–] panathea@lemmy.blahaj.zone 15 points 1 day ago

I switched to subq from IM because of the soreness. It's normal, though.

It just took switching to shorter needles and injecting into pinched fat on my abdomen.

[–] 0x2640@lemmy.blahaj.zone 7 points 1 day ago (3 children)

spoilermuscle soreness is pretty normal with IM injections (seeing as ur injecting into well... muscle).

i recommend using a smaller needle (25g or higher) to draw as it minimizes coring risk, you could just use the needle youre using to inject to do it. swapping needles isnt really necessary, see insulin syringes (but theres also not really a reason to not do it).

it is verrrrrry recommended to inject into your thigh, injecting into your glute carries a high risk of accidentally hitting your sciatic nerve (bad time) and its also just less visibility. no being underweight doesnt mean you cant inject to your thigh.

other than that, you could always switch to subq rather than IM, as levels between the two are functionally identical on cypionate, which may help with the soreness.

[–] LadyMeow@lemmy.blahaj.zone 2 points 14 hours ago (1 children)

I draw with 18 and inject with… currently 25, but have used 22, 23, 27 as well.

If you have the needles to switch, I would. Even one use like poking the rubber will cause some wear on the needle, and thus a slightly worse insertion.

[–] 0x2640@lemmy.blahaj.zone 2 points 10 hours ago

well drawing with 18 is reallllly not recommended with vials you are planning to use more than a few times but like, you do you :p the risk of coring is demonstrably, factually higher (and thats a bad thing)

[–] Catoblepas@piefed.blahaj.zone 6 points 1 day ago (2 children)

it is verrrrrry recommended to inject into your thigh, injecting into your glute carries a high risk of accidentally hitting your sciatic nerve

Is this true for subq as well as IM? Because if so that might explain why some of my shots went so badly 😅

You could also use your stomach for subQ. There's also a spot on your arm that could be used if you have someone else injecting for you. I alternate which thigh I use each week for E and use my stomach rotating which quadrant I inject into for an unrelated injection (that uses an autoinjector).

[–] mossy_@lemmy.world 3 points 1 day ago* (last edited 1 day ago) (1 children)

that's really good to know 😬 I'll stick to the frontside then. Means I have less improvised yoga to do anyway.

I'm underweight but I have legs like a, uh, someone who does a lot of walking. So I've got enough muscle to inject in the front of the thigh, but probably not enough for subcutaneous.

[–] 0x2640@lemmy.blahaj.zone 1 points 1 day ago

im extremely underweight, i can still do subq :p itll be fine (and even if you end up doing IM accidentally, itd be with a smaller and shorter needle, so less owie anyway)

[–] onslaught545@lemmy.zip 2 points 1 day ago* (last edited 1 day ago) (1 children)

You don't need a 1.5in needle for an IM injection if you're underweight. I use a 1in 23g for my testosterone without any issue. Injection site muscle soreness is a common side effect of IM injections, so you're fine.

Do it in your outer thigh next time, and don't insert the needle completely perpendicular to your leg. You want it like 15° off vertical with the flat portion of the needle tip facing your leg. Make sure to rub the spot well after the injection too.

[–] 0x2640@lemmy.blahaj.zone 2 points 1 day ago (2 children)

You want it like 15° off vertical with the flat portion of the needle tip facing your leg.

this goes against... how any intramuscular injection is done, and does not sound like sound medical advice to me. any medical textbook, government website, or internet source will tell you its a 90 degree angle (vertical).

[–] onslaught545@lemmy.zip 1 points 1 day ago* (last edited 1 day ago)

Going in at a slight angle helps with the pain from the needle breaking the skin, especially if you need to use a larger gauge needle for thick liquids like testosterone.

I'm not talking about a SQ angle, just barely not a 90° angle. It's not going to change the depth a meaningful amount, especially if you're using a 1.5" tip.

This sounds like subcutaneous injection instructions.

[–] clay_pidgin@sh.itjust.works 2 points 1 day ago (1 children)

Hi, here from /all. What part is DIY? Did you make your own medicine, do you not have a prescription, or is it common to need a doctor to inject you?

[–] ElfBean@fedia.io 10 points 1 day ago (1 children)

TLDR; obtaining and administering all necessary medications and blood tests yourself. Not everyone is willing or able to go through medical professionals for any number of reasons, mainly costs and medical gatekeeping. When done right the risks are minimal, though never nonexistent ofc.

[–] clay_pidgin@sh.itjust.works 4 points 1 day ago (3 children)

Makes sense! If you're aware of the risks (I have no idea what they might be) then I think it's your business. I was imagining OP in the kitchen with a Bunsen burner and a smoking Erlenmeyer flask!

[–] hedgehog@ttrpg.network 5 points 1 day ago

Now I’m imagining Breaking Bad but making E instead of Meth.

[–] WalrusDragonOnABike@reddthat.com 2 points 1 day ago (1 children)

Some people do start with raws and do sterilization while missing with the oils for doing the injection, which involves some extra equipment (although some people may already have it lying around in their kitchen). Self-injection is the norm AFAIK.

[–] clay_pidgin@sh.itjust.works 1 points 1 day ago

Thank you for the detail.

[–] mossy_@lemmy.world 2 points 1 day ago

dude I wish. amateur alchemy might be in the future for me but for now I'm taking whatever help I can get